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Pyrrolysine—tRNAPyl ligase

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Pyrrolysine—tRNAPyl ligase
Identifiers
EC no.6.1.1.26
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
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NCBIproteins

Pyrrolysine—tRNAPyl ligase (EC 6.1.1.26, PylS, pyrrolysyl-tRNA synthetase) is an enzyme with systematic name L-pyrrolysine:tRNAPyl ligase (AMP-forming).[1][2][3] This enzyme catalyses the following chemical reaction

ATP + L-pyrrolysine + tRNAPyl AMP + diphosphate + L-pyrrolysyl-tRNAPyl

This enzyme is specific for pyrrolysine as substrate as it cannot be replaced by lysine or any of the other natural amino acids.

References

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  1. ^ Blight SK, Larue RC, Mahapatra A, Longstaff DG, Chang E, Zhao G, Kang PT, Green-Church KB, Chan MK, Krzycki JA (September 2004). "Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo". Nature. 431 (7006): 333–5. doi:10.1038/nature02895. PMID 15329732. S2CID 4395541.
  2. ^ Polycarpo C, Ambrogelly A, Bérubé A, Winbush SM, McCloskey JA, Crain PF, Wood JL, Söll D (August 2004). "An aminoacyl-tRNA synthetase that specifically activates pyrrolysine". Proceedings of the National Academy of Sciences of the United States of America. 101 (34): 12450–4. doi:10.1073/pnas.0405362101. PMC 515082. PMID 15314242.
  3. ^ Schimmel P, Beebe K (September 2004). "Molecular biology: genetic code seizes pyrrolysine". Nature. 431 (7006): 257–8. doi:10.1038/431257a. PMID 15372017.
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