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File:Site-directed mutagenesis library cloning steps.svg

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English: Depiction of one common way to clone a site-directed mutagenesis library (i.e., using degenerate oligos). The gene of interest is PCRed with oligos that contain a region that is perfectly complementary to the template (blue), and one that differs from the template by one or more nucleotides (red). Many such primers containing degeneracy in the non-complementary region are pooled into the same PCR, resulting in many different PCR products with different mutations in that region (individual mutants shown with different colors below).
Date
Source Own work
Author Rob Hurt

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Site-directed mutagenesis library cloning steps

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19 April 2020

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current19:14, 19 April 2020Thumbnail for version as of 19:14, 19 April 2020512 × 284 (19 KB)Rob HurtUploaded own work with UploadWizard

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